Cameron Bancroft will replace the middle-order batsman for the three-day match against the Men in Green
Middle-order batsman Nic Maddinson on Saturday pulled out of Australia A's clash against Pakistan in Perth on mental health grounds, all-but ruling him out of contention for a berth in the Test squad.
The middle-order batsman's decision comes barely a week after all-rounder Glenn Maxwell withdrew from Australia's Twenty20 team to deal with similar issues.
Opener Cameron Bancroft will replace Maddinson for the three-day match against Pakistan at Perth Stadium from Monday.
"Nic has made the right decision and we are all behind him," said Australia A coach Graeme Hick. "It is braver to speak up than to suffer in silence and I applaud Nic for having the courage to put his health first."
His withdrawal almost certainly rules him out of the opening Test against Pakistan in Brisbane later this month, with the Australia A game widely seen as a final chance to impress selectors.
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It opens the door to left-hand batsman Travis Head or rising star Will Pucovski, who has also struggled with mental health issues in the past, to stake their case.
Maddinson's decision followed Maxwell late last month opting to take a "short" break from cricket to deal with similar challenges.
Alex Kountouris, Cricket Australia's head of sports science and medicine, said the governing body was committed to better understanding mental health and supporting those needing help.
"Mental health is a challenge faced by Australian communities and elite sporting organisations and cricket is no different," said Kountouris. "Like other professional sports we are working very hard to better understand the challenges faced by our players and staff so we can support them."